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. Author manuscript; available in PMC: 2013 Dec 27.
Published in final edited form as: Oncogene. 2012 Jul 23;32(26):3198–3206. doi: 10.1038/onc.2012.325

Figure 4. TGFβ represses the transcriptional activity of the LPA1 gene promoter containing TIEs.

Figure 4

A. DNA sequences of two potential TIEs of the human LPA1 promoter were compared with that of the c-myc TIE (upper). The potential Smad, E2F4/5 and ATF3 binding sites were indicated. The LPA1 promoter (-1156 to +86) was cloned into pGL2-Basic-Luc to constructed the pGL2-LPA1-Luc luciferase reporter (WT) (lower). The deletion (del) and point mutations of each TIE (-401 Mut and -40 Mut) were made as described in Materials and Methods. B. MDA-MB-231 and SKOV-3 cells were transfected with the indicated plasmids and cultured with TGFβ or vehicle for 16 hours before luciferase activities were determined. The results were presented as percentages relative to the values of the vehicle control cells (defined as 100%).