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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Neurobiol Aging. 2012 Jul 20;34(3):943–960. doi: 10.1016/j.neurobiolaging.2012.06.010

Figure 6. Effects of intracellular A2E on microglial expression of chemokines/chemokine receptors and microglial chemotaxis.

Figure 6

(A) Changes in mRNA expression of chemokine receptors following 6 hours of incubation in 6 μM of A2E were assayed using RT-PCR. Gene expression levels of CX3CR1, CCR2, CCR5, and CCR1 were also lowered relative to non-loaded control microglia. (Data are the mean ± SEM, n = 3, *indicates comparisons for which p<0.05 relative to control, unpaired t-test.) (B) Chemotaxis of cultured retinal microglia with and without A2E loading were also assessed using a Boyden chamber assay using CCL2 (100ng/ml) (upper) and CCL3 (50ng/ml) (lower) as chemoattractants. A2E-loading of microglia induced a dose-dependent decrease in chemotaxis relative to controls (microglia not preloaded with A2E). (Data comprise of mean ± SEM, n = 6, *indicate comparisons for which p<0.05, 1-way ANOVA with Tukey-Kramer multiple comparison test.)