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. 2012 Jul 16;41(4):1387–1396. doi: 10.3892/ijo.2012.1556

Figure 3.

Figure 3

NNK-induced gene and protein expression in H23 and BEAS-2B. Gene expression fold change in cell lines treated with NNK (100 nM) vs. control in (A) human lung adenocarcinoma cells H23 and (B) normal lung epithelial cells BEAS-2B. The gene expression was normalized with endogenous control gene UBC. An asterisk above a bar indicates significant (P<0.05) differential expression in repeated ANOVA tests of five biological samples and three technical repeats in qRTPCR assays. Protein expression measured by western blots in NNK treated cell lines (C and D) BEAS-2B and H23 for EGFR, (E and F) p53, (G and H) phospho-p53 and (I and J) Rb. The protein expression was quantified with densitometry and normalized with endogenous control protein GAPDH in three biological repeats.