Figure 3.

Spermidine treatment promotes resistance to 1% H₂O₂ both in normal and in autophagy-deficient Drosophila. (a and b) Survival curve for female (a) and male (b) wild-type flies exposed to 1% H₂O₂ and treated with either no spermidine (control), 0.01, 0.1 or 1 mM spermidine (spd.). Results represent the pooled data of three independent replicate experiments. (c and d) Survival curve for female (c) and male (d) atg7^−/− flies exposed to 1% H₂O₂ and treated with either no spd. (control), 0.01, 0.1 or 1 mM spd. Results represent the pooled data of three independent replicate experiments. (e) Relative mean survival±S.E.M. for female and male wild-type flies exposed to 1% H₂O₂ and treated with either no spd. (control), 0.01, 0.1 or 1 mM spd. Mean survival was normalized to the untreated control. Results represent the pooled data of three independent replicate experiments (***P<0.001). (f) Relative mean survival±S.E.M. for female and male atg7^−/− flies exposed to 1% H₂O₂ and treated with either no spd. (control), 0.01, 0.1 or 1 mM spd. Mean survival was normalized to the untreated control. Results represent the pooled data of three independent replicate experiments (***P<0.001)