Figure 5.

Double staining with phalloidin (green) and anti-Arp2/anti-Arp3/anti-Mena/anti-Vasp antibodies (red) in vehicle-treated NSCLC (A549) cells and DAMTC-treated NSCLC (A549) cells. The Arp2, Arp3 and Vasp can be clearly observed along with phalloidin (merging of green and red) in the membrane protrusions, whereas the Mena protein is concentrated in the cytosol and absent from the membrane protrusions in DAMTC-treated cells. The blue dye represents the DAPI-stained nucleus. The corresponding localization of these proteins is also shown for the vehicle-treated NSCLC (A549) cells