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. 2012 Oct 15;63(17):6211–6222. doi: 10.1093/jxb/ers273

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© 2012 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Analysis of BnCIPK6 promoter activity in Arabidopsis plants under NaCl, mannitol, and abscisic acid (ABA) treatments. 12-d-old transgenic Arabidopsis seedlings containing the BnCIPK6 promoter fused to the GUS reporter gene were treated without (control) or with 150mM NaCl, 300mM mannitol, and 100 µM ABA for 6h. (a) A control seedling; (b) a seedling with NaCl treatment; (c) a seedling with mannitol treatment; (d) a seedling with ABA treatment. (e) Measurement and quantitative analysis of GUS activity in BnCIPK6p:GUS transgenic Arabidopsis plants under NaCl, mannitol or ABA treatments. Mean values and standard errors (bar) were shown from three independent experiments. Independent t tests for equality of means demonstrated that there was very significant difference between CK and NaCl-, mannitol-, or ABA-treated transgenic plants (**P value <0.01).