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. 2012 Oct 15;63(17):6211–6222. doi: 10.1093/jxb/ers273

Fig. 4.

Fig. 4.

Over-expression of BnCIPK6 and BnCIPK6M in Arabidopsis enhances plant tolerance to salt stress. (a) In vitro phosphorylation assay of recombinant MBP-BnCIPK6 and MBP-BnCIPK6M fusion proteins. (b) Quantitative analysis of autophosphorylation activities of BnCIPK6 and BnCIPK6M. (c) Quantitative RT-PCR analysis of BnCIPK6 and BnCIPK6M expression in transgenic Arabidopsis. (1) WT; (2) PMD vector; (3–6) BnCIPK6M transgenic lines L5, L8, L9, and L10; (7–9) BnCIPK6 transgenic lines L1, L3, and L6. (d) One-week-old seedlings were transferred and grew for 5 d on MS medium supplemented with 170mM NaCl. (e) Statistical analysis of chlorophyll content of leaves of seedlings grown on MS medium containing 170mM and 200mM NaCl for 7 d. (f) Measurement of proline content in seedlings of wild-type and transgenic lines treated with 170mM and 200mM NaCl for 24h. Mean values and standard errors (bar) were shown from three independent experiments (n >50 seedlings per each line). Independent t tests for equality of means demonstrated that there was very significant difference between wild type and transgenic plants (**P value <0.01). (This figure is available in colour at JXB online.)