Fig. 6.

Over-expression of BnCIPK6, BnCIPK6M, and BnCBL1 in Arabidopsis enhances plant tolerance to phosphorous starvation. (a–c) Transgenic Arabidopsis plants over-expressing BnCIPK6M and BnCIPK6 were transferred and grown for 9 d on MS medium and low phosphate medium. (a) Six-day-old seedlings were transferred and grown on MS medium. (b) Six-day-old seedlings were transferred and grown on MS medium with 50 µM phosphate (low phosphate, LP). (c) Statistical analysis of the lateral root number. (d–f) Transgenic Arabidopsis plants over-expressing BnCBL1 transferred and grown for 9 d on MS medium and low-phosphate medium. (d) One-week-old seedlings were transferred and grown on MS medium as controls. (e) One-week-old seedlings were transferred and grown on low- phosphate medium (50 µM Pi). (f) Statistical analysis of plant fresh weight. Mean values and standard errors (bar) were shown from three independent experiments (n >50 seedlings per each line). Independent t-tests for equality of means demonstrated that there was significant difference (*P value <0.05) or very significant difference (**P value <0.01) between wild-type and transgenic plants. (This figure is available in colour at JXB online.)