Table 3.

Comparison of the proposed key amino-acid residues found in the predicted active sites of AtCAD5, PotSAD, BdCAD3, and BdCAD5

AtCAD position	AtCAD5	BdCAD5	PotSAD	BdCAD3	Notes
49	T	T	S	T	NADP-binding/ H-shuttle
52	H	H	H	H	NADP-binding
53	Q	Q	S	I	Substrate-binding
57	D	H	D	E	H-shuttle
58	L	L	W	W	Substrate-binding
60	M	A	F	N	Substrate-binding
70	E	E	E	E	Zn-binding
95	C	V	C	Y	Substrate-binding
119	W	W	L	L	Substrate-binding
192	V	V	L	L	NADP-binding
211	S	S	S	S	NADP-binding
212	S	S	T	S	NADP-binding
213	S	S	S	S	NADP-binding
216	K	K	K	K	NADP-binding
276	V	V	A	A	Substrate-binding
286	P	P	F	Y	Substrate-binding
289	M	M	I	I	Substrate-binding
290	L	L	A	T	Substrate-binding
299	F	F	G	G	Substrate-binding
300	I	I	I	V	Substrate-binding
Ratio to AtCAD5	20/20	17/20	7/20	7/20

Residues were selected based on the published structures of AtCAD5 and PotSAD.