Table 1.
Fold change of transcripts coding for sulphur-rich proteins in SMARC1N-PN1 as compared with SARC1. A negative value refers to a reduced expression in SMARC1N-PN1 by the corresponding fold change. Values statistically significant at P ≤0.05 are highlighted in bold. Contigs with evidence for differential expression at the protein level are highlighted with an asterisk (Marsolais et al., 2010; Yin et al., 2011). Protein accession was identified by BlastX of early release of the Phaseolus vulgaris genome (Phaseolus vulgaris v1.0, DOE-JGI and USDA-NIFA, http://www.phytozome.net/commonbean_er).
| Annotation | Contig/singleton | Protein | Identity (%) | Developmental stage | |||
|---|---|---|---|---|---|---|---|
| IV | V | VI | VIII | ||||
| Legumin | CL647Contig1* | Phvul.007G192800.1 | 98 | 1.46 | 2.33 | 1.35 | 1.70 |
| Basic 7S globulin-2 | CL10519Contig1 | Phvul.006G093600.1 | 100 | 1.17 | 2.20 | 1.80 | 2.25 |
| CL671Contig1 | Phvul.006G093600.1 | 100 | 1.11 | 2.49 | 2.15 | 2.02 | |
| Albumin-2 | CL46Contig1* | Phvul.007G275800.1 | 100 | 2.60 | 2.46 | 1.23 | 1.28 |
| CL46Contig3 | Phvul.007G276200.1 | 100 | 1.10 | 1.17 | 1.00 | 2.44 | |
| CL46Contig7 | Phvul.007G276200.1 | 88 | 1.08 | 1.22 | −1.09 | 2.27 | |
| CV533282 | Phvul.007G276200.1 | 91 | −1.14 | −1.11 | –1.66 | 2.89 | |
| FG231390 | Phvul.007G276100.1/-.2 | 91 | 1.22 | 1.37 | 1.12 | 2.68 | |
| Defensin D1 | CL586Contig1* | Phvul.005G071300.1 | 99 | 1.17 | 1.43 | 3.86 | 1.03 |
| γ-Thionin | CL1108Contig1 | Phvul.009G158000.1 | 100 | 1.38 | 2.03 | 3.15 | 1.68 |
| Albumin-1A | CL1Contig108* | Phvul.011G205400.1/-300.1 | 97 | 1.44 | 1.38 | 1.40 | 9.77 |
| Albumin-1A | PVUSE3NG_T3_011_O21 | Phvul.011G205400.1/-300.1 | 96 | 1.07 | 1.06 | 1.08 | 4.00 |
| Albumin-1A | CL1Contig69 | Phvul.011G205400.1/-300.1 | 90 | 1.19 | 1.32 | 1.25 | 5.58 |
| Albumin-1B | CL1Contig83* | Phvul.011G205400.1/-300.1 | 98 | 1.33 | 1.09 | 1.13 | 4.77 |
| Albumin-1B | CL1Contig10* | Phvul.011G205400.1/-300.1 | 94 | 1.39 | −1.01 | 1.00 | 4.66 |
| Albumin-1B | CL1Contig64 | Phvul.011G205400.1/-300.1 | 92 | 1.70 | 1.30 | 1.64 | 6.85 |
| Albumin-1C | CL1Contig43 | Phvul.011G204600.1 | 93 | 1.84 | 1.89 | 1.38 | 2.04 |
| Albumin-1C | CV539755 | Phvul.011G204600.1 | 91 | 2.48 | 1.35 | 1.35 | 1.35 |
| Albumin-1C | CV538174 | Phvul.011G204600.1 | 88 | 2.27 | 2.15 | 1.58 | 2.30 |
| Albumin-1C | CV540424 | Phvul.011G204700.1 | 88 | 3.00 | 2.50 | 1.50 | 1.42 |
| Albumin-1D | CL1Contig82 | Phvul.011G205500.1 | 99 | 1.87 | 1.20 | 1.38 | 8.38 |
| Albumin-1D | CL1Contig87 | Phvul.011G205500.1 | 99 | 1.46 | 1.12 | 1.00 | 5.55 |
| Albumin-1D | PVUSE4NG_RP_1004_F21 | Phvul.011G205500.1 | 95 | 1.28 | 1.03 | −1.01 | 3.10 |
| Albumin-1D | PVUSE2NG_RP_027_K19 | Phvul.011G205500.1 | 93 | 1.34 | 1.10 | 1.05 | 6.29 |
| Albumin-1E | CL1Contig17 | Phvul.011G204700.1 | 99 | 7.17 | 3.39 | 2.37 | 5.62 |
| Albumin-1E | CL1Contig141 | Phvul.011G205200.1 | 100 | 1.37 | 2.33 | 1.14 | 1.37 |
| Albumin-1F | CL1Contig110 | Phvul.011G205600.1 | 100 | 1.67 | 1.07 | 1.70 | 10.21 |
| Albumin-1F | PVUSE2NG_RP_018_O15 | Phvul.011G205500.1 | 95 | 1.30 | 1.64 | 2.54 | 10.88 |
| Albumin-1F | PVUSE3NG_RP_014_O07 | Phvul.011G205400.1/-300.1 | 94 | 1.21 | 1.02 | 1.25 | 4.64 |
| Albumin-1G | PVUSE4NG_RP_1003_G20 | Phvul.011G205500.1 | 100 | 1.72 | 1.18 | 1.37 | 5.64 |
| Albumin-1G | CL1Contig42 | Phvul.011G204400.1 | 98 | 2.18 | 1.61 | −1.20 | 1.98 |
| Bowman–Birk type proteinase inhibitor 2 | CL1Contig140* | Phvul.004G133900.1 | 100 | 3.80 | 3.58 | −1.03 | 1.30 |
| Bowman–Birk type proteinase inhibitor 2 | PVUSE4NG_RP_1003_O21 | Phvul.004G133900.1 | 100 | 4.28 | 3.58 | 1.04 | 1.30 |
| Double-headed trypsin inhibitor | CL10Contig1 | Phvul.004G134100.1 | 95 | 3.19 | 1.71 | −1.03 | 1.09 |
| Trypsin/chymotrypsin inhibitor | CL10Contig2 | Phvul.004G134000.1 | 95 | 3.21 | 1.78 | 1.03 | 1.04 |
| Trypsin/chymotrypsin inhibitor | PVUSE3NG_RP_1003_M20 | Phvul.004G134000.1 | 89 | 3.19 | 2.36 | 1.01 | 1.53 |
| Kunitz trypsin protease nhibitor | CL3713Contig1 | Phvul.004G129900.1 | 88 | −1.25 | −1.19 | 2.06 | 3.00 |
| Trypsin inhibitor | PVUSE2NG_RP_008_J18 | Phvul.004G129900.1 | 81 | −1.31 | −1.23 | 3.05 | 3.48 |