TABLE 1.
Primers used for mutagenesis
| Mutation | Template | Primer (forward, 5′-3′) |
|---|---|---|
| M134S | MoPrP | ATGCTGGGGAGCGCCAGTAGCAGGCCCATGATC |
| M154S | MoPrP | TACTACCGTGAAAACAGTTACCGCTACCCTAAC |
| M205S | MoPrP | ACCGATGTGAAGATGAGTGAGCGCGTGGTGGAG |
| M212S | MoPrP | CGCGTGGTGGAGCAGAGTTGCGTCACCCAGTAC |
| M205S,M212S | MoPrP M205S | CGCGTGGTGGAGCAGAGTTGCGTCACCCAGTAC |
| M134S | HaPrP | CTGGGGAGTGCCTCCTCTAGACCCATG |
| HaPrP-YFP | ||
| M154S | HaPrP | CTACCGTGAAAACTCCAATCGATACCC |
| HaPrP-YFP | ||
| M206S | HaPrP | GACATCAAGATATCCGAGCGCGTGG |
| HaPrP-YFP | ||
| M213S | HaPrP | GTGGTGGAGCAGTCCTGTACCACCCAG |
| HaPrP-YFP | ||
| M206S,M213S | HaPrP M206S | GTGGTGGAGCAGTCCTGTACCACCCAG |
| HaPrP-YFP-M206S | ||
| M213L | HaPrP | GTGGTGGAGCAGCTCTGTACCACCCAG |
| HaPrP-YFP | ||
| A117V | HaPrP | GCCGGCGCTGCTGTGGCAGGGGCC |
| HaPrP-YFP | ||
| C214A | HaPrP | GAGCGCGTGGTGGAGCAGATGGCTACCACCCAGTATCAG |
| HaPrP-YFP | ||
| G123P | HaPrP | GCCGTGGTGCCGGGCCTTGGTG |
| HaPrP-YFP | ||
| HaPrPDS | ||
| HaPrP- YFPDS |