FIGURE 3.

Overexpression of mEpo-R in human primary erythroid progenitor cells. A, cell proliferation (left) and % benzidine positive cells (right) following 12 days of treatment with EPO in primary adult erythroid progenitor cell (hAEPC) cultures with (mEpoR-transfected) and without (Mock-transfected) forced mEPO-R expression are shown. B, expression of endogenous TAL1, EPO-R, β-globin, EKLF, GATA-1, and GATA-2 were monitored during 12 days of EPO stimulation with (triangle, solid line) and without (square, dashed line) overexpression of mEPO-R in hAEPC. C, expression of γ-globin and the γ-globin/(γ-globin + β-globin) ratio (γ/(γ + β)) were monitored during EPO stimulation with (triangle, solid line) and without (square, dashed line) overexpression of mEpo-R in hAEPC. D, EPO-R overexpression was assessed and normalized to β-actin using Western blotting. Results are normalized to β-actin expression. Data represent mean ± S.D. *, p < 0.05.