TABLE 2.

Thermodynamic parameters of the bindings of LGN GoLoco motifs to Gα_i3·GDP determined by ITC titration

The titration data of GL12 and GL123 were fitted with the two sets of binding sites model, whereas the other data were fitted with the one set of binding sites model. N denotes the number of binding sites in each model.

	N	KD	ΔH	ΔS	ΔG
		nm	kcal mol−1	cal mol−1 K−1	kcal mol−1
GL12
Site 1	0.97	11.27	−16.69	−18.7	−11.12
Site 2	0.89	188.32	−22.80	−44.4	−9.57
GL123
Site 1	1.63	4.69	−12.55	−3.97	−11.37
Site 2	1.0	186.22	−15.46	−21.0	−9.20
GL23	1.86	87.72	−17.05	−24.0	−9.90
GL34	1.81	173.61	−20.97	−37.2	−9.88
GL234	3.08	358.42	−5.12	11.9	−8.67