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. 2012 Aug 10;287(44):36814–36829. doi: 10.1074/jbc.M112.356048

FIGURE 2.

FIGURE 2.

CaMKII activates Notch1 transcriptional activity. A–C, HEK293 cells were transfected with 50 ng of 4XCSL-Luc (A), 50 ng of Hes1-Luc (B), 50 ng of Hes5-Luc and RMHes5-Luc (C), and 50 ng of β-galactosidase, along with 100 ng of Notch1-IC and 50 ng (+), 100 ng (++) and 150 ng (+++) of CaMKII, as indicated. D–F, HEK293 cells were transfected with 50 ng of 4XCSL-Luc (D), 50 ng of Hes1-Luc (E), 50 ng of Hes5-Luc (F), and 50 ng of β-galactosidase, along with 50 (+), 100 (++), and 150 ng (+++) of CaMKII, as indicated. 48 h after transfection, the cells were lysed, and the luciferase activity was determined. *, p < 0.01 (A and C) or p < 0.05 (B) versus Notch. D–F, *, p < 0.05 versus control. R.L.U., relative luciferase units.