TABLE 1.
C. albicans and S. cerevisiae strains and oligonucleotides used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| S. cerevisiae strains | ||
| W303-1A | MATa leu2–3, 112 ura3–1, trp1–92, his3–11, 15 ade2–1, can1–100 GAL, SUC2, MAL | 49 |
| LK41 | W303-1A gpr1Δ::URA3 | 9 |
| JS4 | W303-1A gpr1Δ::URA3 + CaGPR1int | This study |
| JS16 | W303-1A gpr1Δ::URA3 + ScGPR1int | This study |
| C. albicans strains | ||
| CAI4+URA | URA3/ura3Δ::λimm434 | 50 |
| HT10 | URA3/ura3Δ::λimm434 gpr1Δ::hisG/gpr1Δ::hisG | This study |
| LR2 | ura3Δ::λimm434/ura3Δ::λimm434 GPR1-URA3 /gpr1Δ::hisG | 7 |
| BSC7 | URA3/ura3Δ::λimm434 cdc25Δ::cmLEU2/cdc25Δ::cdHIS1 | This study |
| CR216 | ura3Δ::λimm434/ura3Δ::λimm434 | 8 |
| cdc35Δ::hisG/cdc35Δ::hisG-URA3-hisG | ||
| BSI3 | URA3/ura3Δ::λimm434 | This study |
| ira2Δ::FRT/ira2Δ::FRT | ||
| Oligonucleotides | ||
| pCaGPR1tF | GCAGTAAGAGTCACCAAAAAAAAAAAACGACAAACAAGTGATCCGAAGTGTGACGAATAAAGCAAACTCTCCAACTCAAAATGCCGGACCTAATATCAATAGC | |
| pCaGPR1tR | GAGCGRCATTCATGTCGGCTACTTGTCAATTTGTATTACGTTCCTTACTTTCCATTTTCAAACATCGCGATACAAAAACTTTACATTGGGGGTCTTTTTGAG | |