FIGURE 5.

CHP3-mediated oligosaccharide maturation of NHE1 does not require N-myristoylation or Ca²⁺ binding. AP-1 cells were transiently cotransfected with equal quantities of NHE1_HA and empty vector or variants of CHP3_myc (WT, G2A, and D123A). Cell lysates were prepared at the indicated time points following transfection (0–72 h) and subjected to SDS-PAGE and immunoblotting to detect expression of NHE1_HA and CHP3_myc as described in the legend to Fig. 4. Blots were stripped and reprobed for expression of endogenous GAPDH as a control for protein loading. Data shown are representative of three independent experiments. cg, core-glycosylated; fg, fully glycosylated.