FIGURE 5.

PGE₁OH induces CREB and C/EBP. A, purified CD11c + DC were stimulated with TNFα in the presence or absence of PGE₁OH for 1 h. Nuclear extracts were prepared and subjected to a protein/DNA array as described under “Experimental Procedures.” B, the nucleotide sequence for the IL-23p19 promoter region (NCBI reference sequence) is shown. Putative transcription factor binding sites (boxed-in sequences) were identified by using predictive algorithm-based programs listed under “Experimental Procedures.” Underlined sequences refer to binding sites for transcription factors previously described for IL-23p19 regulation.