FIGURE 9.

Effect of endogenous PGE2 on IL-23p19 expression. DC were pretreated for 30 min with indomethacin (1 mm). Cells were stimulated with LPS (1 μg/ml) or (A) TNFα (100 ng/ml) (B) in the presence or absence of PGE₁OH (1 μm) for 3 h. Expression levels of IL-23p19 and Cox2 were determined by RT-PCR. ** indicates p < 0.01 compared with LPS alone, LPS + PGE₁OH, and TNFα + PGE₁OH. N.S., not significant. Data are representative of two independent experiments.