FIGURE 2.

Down-regulation of GSK leads to increased WC-1 levels. A, QA titration: Western blots showing levels of GSK, WC-1, WC-2, and FRQ in the qa-gsk and wt ctrl strains from cultures grown at 25 °C in light at the indicated QA concentrations. A Ponceau stain of the blot is shown as a loading control. B, down-regulation of GSK affects WC-1 independent of light. Representative Western blots showing levels of WC-1 and GSK in qa-gsk grown in darkness for 30 h (DD30) in the presence or absence of 1.5 × 10⁻³ m QA. As a loading control the blot was additionally decorated for tubulin. C, Western blot showing that light induced hyperphosphorylation of WC-1 is not affected when GSK levels are low. Cultures grown in the presence or absence of QA in constant darkness for 24 h (DD24) were transferred to light and harvested after 15 min (LI 15′). Black arrowheads indicate hyperphosphorylated WC-1 species.