WC-1 levels are elevated in gskhet in a post-transcriptional manner.
A, representative Western blots showing levels of clock proteins in the gskhet strain compared with its background control strain wt9718 at steady state conditions (LL, 30 °C). B, quantification of WC-1, WC-2, and FRQ protein (left panels) and mRNA (right panels) from at least three independent experiments are shown (± S.E.). wt9718 RNA and protein levels were used as reference. Differences between strains were analyzed using two-tailed Student's t-test, and p values ≤0.05 are indicated with *.