Table I.
Cell-to-cell trafficking of CMV 3a MP:GFP fusion protein in the epidermis of tobacco
| Leaf No. | Leaf Section | 3a MP:GFP | 3a MP (M5):GFP | GFP |
|---|---|---|---|---|
| Onea | 2 /174 | 0 /40 | 0 /200 | |
| Two | Base | 0 /34 | –b | – |
| Tip | 9 /20 | – | – | |
| Three | Base | 14 /25 | 0 /53 | 0 /75 |
| Tip | 7 /13 | – | – |
The proteins (fusion or nonfusion) were produced in planta after plasmids containing the respective genes were delivered into the tobacco epidermis by a particle gun. Three to four leaves from different plants were used in each experiment, except for the first leaf, for which 10 samples were used. The data are the number of cells permitting cell-to-cell trafficking of a protein over the total cells producing the protein.
a
For the first leaf, identical results were obtained when the genes were bombarded into either the tip or the base regions, so the data were pooled.
b
–, Not done.