TABLE 1.
GdA-induced IL-6 secretion by monocytes/macrophages
Monocytes, THP-1 cells, and macrophages (5 × 105) were incubated with 0.1, 1, or 10 μg/ml GdA for 48 h. IL-6 secretion into the culture medium was measured by ELISA (n = 8). IL-6 secretion into the culture medium of monocytes and THP-1 cells was measured after LPS activation. Data are the mean ± S.E.
| IL-6 level |
|||
|---|---|---|---|
| Monocytes | THP-1 cells | Macrophages | |
| pg/ml | |||
| Control | 654.6 ± 265.0 | 952.4 ± 370.4 | 4429.7 ± 752.6 |
| GdA (0.1 μg/ml) | 834.7 ± 315.9 | 1118.9 ± 459.4 | 5305.4 ± 909.8 |
| GdA (1 μg/ml) | 871.0 ± 344.2 | 3331.5 ± 507.9 | 6383.7 ± 1148.4 |
| GdA (10 μg/ml) | 1199.4 ± 391.4a | 4802.1 ± 1107.5a | 9213.6 ± 1277.9a |
a p < 0.05 compared with the corresponding control without GdA treatment.