FIGURE 2.

Comparing the structures of ezrin and phospho-mimetic ezrin(T567D) in solution using SANS. A, SANS I(Q) of ^dezrin at 1.75 mg/ml and ^dezrin(T567D) at 1.57 mg/ml. I(Q) values are scaled to show the different scattering curves. The lines are fit to the experimental data when ab initio reconstructs the three-dimenional shapes shown in C and D. The χ² values for the fit are 0.356 and 0.377 for ^dezrin and ^dezrin(T567D), respectively. The Guinier plots and fits are shown in the inset. B, P(r) functions of ^dezrin and ^dezrin(T567D). C and D, ab initio reconstructed three-dimenional envelopes of ^dezrin and ^dezrin(T567D) are docked with the crystal structure of Sfmoesin (Protein Data Bank code 2I1K). The models are generated by averaging 10 models generated by DAMMIN. The largest NSD value (57) with ^dezrin three-dimenional shape is 0.606 and 0.559 for ^dezrin(T567D).