TABLE 1.

Comparing R_g, D_max, and I(0)/c of ^dezrin* and mutants in buffer, PIP₂-bound, and F-actin-bound states

Static light scattering experiments (see supplemental Fig. S3), which measure the absolute molecular mass, show that the fraction of ezrin eluted at 14.6 ml in the Superdex 200 10/300 GL column is monomeric. The parameters listed here are from P(r) function calculations. See supplemental Table S1 for R_g and I(0) obtained from Guinier fit.

	In 20% D2O buffer			In 2.5 mm PIP2 in 20% D2O			Bound to PIP2 and to F-actin in 40% D2O
	Rg	Dmax	I(0)abs/c	Rg	Dmax	I(0)abs/c	Rg	Dmax	I(0)abs/c
	Å	Å	cm2 mg−1	Å	Å	cm2 mg−1	Å	Å	cm2 mg−1
Ezrina	41.2 ± 0.3	140
dEzrin	40.7 ± 0.5	140	0.31 ± 0.01	67.4 ± 1.2	240	0.28 ± 0.01
dEzrin(T567D)	41.0 ± 0.8	140	0.30 ± 0.01	68.0 ± 1.0	240	0.33 ± 0.01	95.2 ± 0.9	300	0.80 ± 0.05
dEzrin(S249D)	41.4 ± 0.7	140	0.32 ± 0.01
dEzrin(S249D/T567D)	42.0 ± 0.9	150	0.31 ± 0.01

^a Data are from SAXS measurements.