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Quantification of the efficiency of deletion of the Hif-1a, Hif-2a, and Vhl genes by analysis of genomic DNA. Primary chondrocytes were isolated from growth plates of newborn Hif-1af/f, Hif-2af/f, and Vhlf/f mice, cultured in a monolayer and transduced on day 1 after plating with adenoviruses producing either β-galactosidase (black columns) or Cre recombinase (gray columns). Genomic DNA was isolated on day 10. Error bars, S.D. ***, p < 0.01.
