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Analysis of the activity of purified recombinant human C-P4H-I in 21% O2 and 1% O2. The amount of enzyme increased 6-fold (6×) in the assays carried out in 1% O2 relative to that present in the reactions carried out in 21% O2. This C-P4H activity assay measures the hydroxylation-coupled decarboxylation of 2-oxo-[1-14C]glutarate.
