FIGURE 1.

Functional epitope of mAb PRC5. A, Western blot reactivity with various PrP primary structures. AHa, Armenian hamster; Sq. Mon., squirrel monkey. B, primary structures are aligned between mouse PrP residues 123 and 163. Reactive species are boxed and shaded red. The 6H4 and D18 epitopes are bold in cattle and mouse PrP, respectively. Residues 132 and 158 are boxed and shaded blue, and side chains are yellow in the tertiary structure. C, Western blots of RK13 cell extracts transfected with the following: Vector, pIRESPuro; Mo wt, mouse PrP; Elk wt, elk PrP; Elk V133, elk PrP with Val at residue 133; Elk S162, elk PrP with Ser at residue 162; Mo V132, mouse PrP with Val at residue 132; and Mo S158, mouse PrP with Sr at residue 158.