FIGURE 5.

Effect of mAb recognition following PrP tertiary structure disruption by deletion mutagenesis. A, scheme showing the various deletion constructs and locations of mAb epitopes. Arrows or brackets indicate which deletions harbor epitope components for the various conformation-dependent mAbs. N-Linked glycan attachment sites at residues 180 and 196 are indicated by green hexagons, and the disulfide bond between cysteine residues 178 and 213 as a red line. B, Western blot reactivity of conformation-dependent and -independent mAbs with PNGase F-treated extracts of RK13 cells expressing PrP deletion constructs.