TABLE 1.

Properties of novel epitopes raised against randomly shuffled chimeric PrP

ND means not determined.

mAb	KD (m)		Epitope characteristics				Effect of mutation on conversion of PrPC to PrPSc
	Elk PrP	Mouse PrP	Constitutive amino acids	Linear/discontinuous	Conformation-dependent	Globular domain	Mutation	Mouse PrP	Elk PrP
PRC mAbs
PRC1	7.0 × 10−7		Gln-90	Linear	No	No	PrP3G	Converts	Inhibits
							PrP2G	Converts	Converts
PRC5	9.1 × 10−7	2.1 × 10−6	Ala-132	Discontinuous	Yes	Yes	A132V	Inhibits	Inhibits
			Asn-158				N158S	Converts	Inhibits
PRC7	1.7 × 10−4	ND	Tyr-154	Discontinuous	Yes	Yes	Y154N	Converts	ND
			Gln-185				Q185E	Inhibits	ND
PRC9		1.2 × 10−5	Tyr-224, Asp-226, Gly-227	Linear	No	No
Previously reported mAbs
6H4			Unknown, within 143–151	Linear	Yes	Yes
D18			Unknown, within 132–156	Discontinuous	Yes	Yes
R1			Unknown, within 224–230	Linear	No	No
HumP			Unknown, within 95–107	Linear	No	No
POM2			Unknown, within 81–89	Linear	No	No