TABLE 1.
Summarized results of object-based analyses of co-localization of Cherry-STIM1 and RyR2S437-YFP
| Treatment | No. of puncta per cella | Punctum area | Degree of co-localizationb |
n | |||
|---|---|---|---|---|---|---|---|
| Geometrical centers-particles coincidence analysis |
Distance between geometrical centers analysis |
||||||
| Cherry-STIM1 (red channel) | RyR2S437-YFP (green channel) | Cherry-STIM1 (red channel) | RyR2S437-YFP (green channel) | ||||
| μm2 | % | % | % | % | |||
| Vehicle | 58.3 ± 16.6 | 0.46 ± 0.04 | 24.2 ± 5.2 | 27.7 ± 6.9 | 21.5 ± 3.5 | 27.3 ± 6.8 | 6 |
| Thapsigargin | 87.8 ± 34.8 | 1.02 ± 0.05c | 79.7 ± 3.6c | 79.7 ± 4.6c | 77.8 ± 2.5c | 68.8 ± 5.6c | 6 |
a Total number in both red and green channels.
b Degree of co-localization was calculated for each channel as a percentage of puncta in the red (green) channel co-localizing with the puncta from the green (red) channel divided by the total number of all puncta from the red (green) channel.
c Differences between means are significant (p < 0.001).