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. 2012 Sep 6;287(44):37282–37295. doi: 10.1074/jbc.M112.408401

FIGURE 4.

FIGURE 4.

Down-regulation of Grhl2 affects expression of its target genes. A, qRT-PCR analysis of the expression levels of Grhl2, its potential targets, and type I epithelial cell marker Pdpn in lung epithelial MLE15 and E10 cells is shown. The data sets were normalized against Gapdh (n = 3). Data represent the mean ± S.D. Immunofluorescence analysis of GRHL2 expression in MLE15 cells (B and C) and in E10 cells (D and E) is shown. F, qRT-PCR expression analysis of Grhl2 in MLE15 cells infected with Grhl2-shRNA V2LMM_20130 (Open Biosystems) or non-silencing-shRNA. Data set was normalized against Gapdh (n = 3). Data represent the mean ± S.D. G, the Western blot shows down-regulation of GRHL2 protein in MLE15 cells by shRNA in three independent transductions. Data were normalized to β-actin. H–I, immunofluorescence analysis of GRHL2 protein expression in MLE15 cells infected with Grhl2 shRNA or non-silencing control is shown. J, by qRT-PCR analysis, we determined that knockdown of Grhl2 in MLE15 cells lowers the expression of its target genes. The data sets were normalized against Gapdh (n = 3). Data represent the mean ± S.D.