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. 2012 Sep 6;287(44):37309–37320. doi: 10.1074/jbc.M112.385229

FIGURE 2.

FIGURE 2.

Actopaxin-dependent matrix degradation requires Src and MMP activity. A,Western blot analysis shows that levels of phospho-Src Y418 are elevated in cells expressing WT and S4D/S8D actopaxin compared with parental and Quint cells. B, plating WT and S4D/S8D cells in the presence of the Src and MMP inhibitors PP2 and GM6002, respectively, results in the inhibition of matrix degradation. Scale bar, 20 μm. C, quantitation of percentage of cells degrading matrix shows that Src or MMP inhibition results in a highly significant decrease in matrix degradation of WT and S4D/S8D cells. D, gelatin zymography of U2OS actopaxin cells shows the levels of secreted MMP2 and MMP9. E, gelatin zymography is quantified. *, p < 0.05; **, p < 0.005; ***, p < 0.0005. Error bars, S.E.