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. 2012 Sep 6;287(44):37340–37351. doi: 10.1074/jbc.M112.384743

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Comparison of gene expression and TG accumulation in Huh7 and Huh.8 cells and the role of C/EBPβ knockdown. A, lipogenic gene expression was analyzed by qPCR in Huh7 and Huh.8 cells, and the data were normalized to RPL13A expression. The values are presented as the means ± S.E. *, p < 0.05 (n = 3–5). B, identical lipogenic gene expression was measured in Huh7 and Huh.8 cells after infection with nontargeting or C/EBPβ shRNA adenoviruses. The values are presented as the means ± S.E. *, p < 0.05 versus Ad-shcntl (n = 3–5). C, Huh7 and Huh.8 cells were infected with nontargeting or AdC/EBPβ shRNA adenoviruses, and TG accumulation was measured as detailed under “Experimental Procedures.” The values are presented as the means ± S.E. *, p < 0.05 versus Huh7 control (n = 2). D, fatty acid oxidation and mitochondrial transcription factor gene expression was analyzed by qPCR in Huh7 and Huh.8 cells as in A and B, and the data were normalized to RPL13A expression. The values are presented as the means ± S.E. *, p < 0.05 versus Huh7 cntl; #, p < 0.05 versus Ad-shcntl (n = 3–5).