FIGURE 2.

Normalized pCa-force relationship in skinned cardiac muscle fibers. Each skinned muscle preparation was treated with HCTnT or HSSTnT to displace the endogenous troponin complex and reconstituted with the HCTnI·HCTnC binary complex. The Ca²⁺ dependence of force development was measured in each preparation after whole troponin complex reconstitution at pH 7.0. Data are plotted as a comparison between HCTnT3 and HSSTnT isoforms, are reported as the average of several experiments, and expressed as mean ± S.E. The inset in the left top panel shows the difference between native and HCTnT3 displaced fibers. Error bars represent S.E.