FIGURE 8.

Determination of the levels of GPx1 and GPx4 following shRNA-mediated silencing. A and B, immunoblots for GPx1 and GPx4 in NIH/3T3 fibroblasts transfected with shRNA plasmids to silence GPx1 and GPx4 as described under “Experimental Procedures.” C, densitometry quantification of results from the immunoblots. Asterisks indicate statistically significant differences in the levels of GPx1 and GPx4 in GPx1 and GPx4 shRNA-transfected NIH/3T3 fibroblasts, relative to the scrambled shRNA (negative control) (Student's t test, unpaired, two-tailed, **, p < 0.001). Values are reported as mean ± range of duplicate determinations.