FIGURE 5.

Immunoprecipitation of VAMP2 and VAMP3. Freshly isolated rat cardiomyocytes were either treated with insulin or oligomycin for 15 min. A, upon lysis, VAMP2 was immunoprecipitated from insulin-treated cardiomyocytes. Subsequently, the immunoprecipitate was blotted against VAMP2, Akt, pAkt, and PKCζ. B, upon lysis, VAMP3 was immunoprecipitated from oligomycin-treated cardiomyocytes. Subsequently, the immunoprecipitate was blotted against VAMP3, AMPK, pAMPK, PKD, and pPKD. Representative Western blots from at least three different experiments (n = 3) are shown. n.a., no antibody during immunoprecipitation (negative control).