FIGURE 5.

PKCϵ mediates the induction of NF-κB-responsive genes. LNCaP cells were treated with either TNFα (10 ng/ml) or vehicle, and RNA was isolated 6 h later for qPCR analysis of COX2, VEGF, MMP9, and IL6. A, effect of RNAi duplexes ϵ1, ϵ2, or NTC transfected 48 h before TNFα treatment. B, effect of the PKCϵ inhibitor ϵV1–2 or Tat carrier (1 μm). C, effect of infection with either PKCϵ or control (LacZ) AdVs (multiplicity of infection = 1 pfu/cell) carried out 24 h before treatment. Results are shown as fold induction over vehicle-treated cells and expressed as mean ± S.E. (n = 3). *, p < 0.05 versus control.