Figure 6.

Experimental validation that miR-7 transfection induces changes in cell adhesion while miR-128 transfection induces changes in cell cycle control. (A) Percent adhesion for miR-7, miR-128 and negative control (CT) transfected Hey cells to basement membrane extract (BME). Cells were labeled with 2 μM Calcein AM for 1 hour and 20,000 labeled cells were seeded in BME coated 96-well plate. Each assay was carried out in triplicate for an adhesion period of 1 hour and 30 minutes. The results demonstrate a significant increase in cell adhesiveness in miR-7 transfected cells relative to negative controls. No significant difference in adhesiveness was observed between miR-128 transfected cells relative to negative controls. Each column represents the mean of three individual experiments. Error Bars = standard deviation. (B) Distribution of miR-7, miR-128 and negative control (CT) transfected Hey cells in specific phases of the cell cycle. MiR-7-transfected cells display a significant increase in the proportion of cells in G0/G1 and a significant decrease in the proportion of cells in the S and G2/M stages of the cell cycle relative to controls. Cells transfected with miR-128 display a significant decrease in the proportion of cells in the G0/G1 and an increase in the proportion of cells in S phase of cell cycle relative to controls. (P-values were determined by ANOVA for each phase of cell cycle for 3 separate transfections. Error bars = standard deviation).