Northern analysis of MT1a and
MT2a expression in developing Arabidopsis seedlings.
Total RNA (10 mg) was extracted from seedlings germinated for 5, 6, 7,
or 8 d in the absence (−) or presence (+) of 40 μm
copper. RNA was separated in a formaldehyde agarose gel, blotted, and
hybridized with digoxigenin-labeled RNA probes. A, Blot probed with
MT1a antisense. B, Blot probed with MT2a
antisense. C, Gel stained with ethidium bromide showing rRNA.