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Northern analysis of MT1a and MT2a expression in developing Arabidopsis seedlings. Total RNA (10 mg) was extracted from seedlings germinated for 5, 6, 7, or 8 d in the absence (−) or presence (+) of 40 μm copper. RNA was separated in a formaldehyde agarose gel, blotted, and hybridized with digoxigenin-labeled RNA probes. A, Blot probed with MT1a antisense. B, Blot probed with MT2a antisense. C, Gel stained with ethidium bromide showing rRNA.
