Figure 3. Re-replication induced from G2 by 3rd thymidine block.

Cells were released from a double thymidine block after prior treatment with either control or geminin RNAi, as in Figure 1A. 14 hr later cells were optionally given a 3rd thymidine block for 10 hr, and then released for a further 5 h. A. Schematic of experiment. B. At different times during the procedure, batches of cells were given a BrdU pulse and then analysed by flow cytometry. Arrows show the movement of cells suggested by the data.