Figure 4. Assessment of cells spontaneously re-replicating from G2.

Cells were released from a double thymidine block after prior treatment with either control or geminin RNAi, as in Figure 1A. 12 hr later cells were pulsed with BrdU for 30 mins. BrdU was then removed and cultures continued for a further 35.5 h. A. Schematic of experiment. B. At different times during the procedure, batches of cells were analysed by flow cytometry.