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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: J Cell Sci. 2012 Feb 24;125(Pt 10):2436–2445. doi: 10.1242/jcs.100883

Figure 5. The G2/M checkpoint promotes re-replication.

Figure 5

Cells were released from a double thymidine block after prior treatment with either control or geminin RNAi, and optionally p53 RNAi, as in Figure 1A. 12 h after release, cells were optionally treated with caffeine, UCN-01 or KU55933 for 6 h. 27 h after thymidine release, cells were pulsed with BrdU and analysed by flow cytometry. A. Schematic of experiment. B. Flow cytometry profiles of geminin RNAi and control RNAi cells plus and minus caffeine treatment. C. Flow cytometry profiles of geminin RNAi and control RNAi cells plus and minus UCN-01 treatment. D. The suppression of re-replication observed in geminin RNAi seen with different inhibitor treatments or with additional p53 knockdown, expressed as a percentage of the amount of re-replication in geminin RNAi cells without inhibitor treatment. E. Demonstration of geminin and p53 knock-down by immunoblotting.