(A) Length and nucleotide positions of PCR amplified and cloned extracellular domain (CD44ECD) and intracellular domain (CD44ICD) are shown. Nucleotide sequence targeted to generate shCD44ECD and shCD44ICD corresponding to exon-2 and exon-10, respectively, are also shown. (B) Immunoblot analysis for CD44 in control, SV, shCD44ECD, shCD44ICD, shCD44ICD + CD44ECD or shCD44ECD + CD44ICD-transfected 4910 and 5310 xenograft cells was performed using a CD44-specific antibody. GAPDH served as a loading control. The experiments were repeated three times and a representative blot is shown.