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Construction of Rop1At promoter:GUS fusion gene. Rop1At genomic clone containing the putative promoter region was cloned into the binary vector as described in the text. Shown is the region joining Rop1At and the GUS gene. The restriction sites used for translational fusion with the GUS gene are shown in bold; underlined sequences are the partial coding region for Rop1At. The last ATG codon shown is the translation-initiation codon for the GUS gene.
