Figure 6.  .

The role of MD-2 in S. marcescens–induced corneal inflammation. (A) Corneas of C57BL/6 and MD-2^−/− mice were abraded and 1 × 10⁷ tobramycin-killed S. marcescens were placed on the corneal surface as before. (B) Corneas of C57BL/6 mice were abraded and 10 μL eritoran tetrasodium (E5564) or vehicle was added for 1 hour prior with a contact lens before stimulation with killed bacteria. After 24 hours, stromal thickness and haze were measured as before, and the number of neutrophils per corneal section was determined after immunostaining with NIMPR14. Data points represent individual corneas, and the experiment was repeated twice with similar results.