Table 1:

The list of 15 DFNB loci screened in this study. The corresponding genes and details of screening markers are shown. Categories and functions of their encoded proteins are mentioned (52)

Locus (gene), Physical location(bp), Category& function	Marker	Physical position (bp)	PCR product range	Forward primer (5′→3′)	Reverse primer (5′→3′)
DFNB1(GJB2, GJB6) GJB2: 20761602..20767114 GJB6: 20796101..20806534) Ion homeostasis proteins: Connexins	D13S1236	22696180–22696305	108–132	GCACTTGGCCTGGGTAA	AAGGGGCTGGCTCTTCA
	D13S1275	-	180–214	ATCACTTGAATAAGAAGCCATTTG	CCAGCATGACCTTTACCAG
	D13S175	20848506–20848618	101–113	TATTGGATACTTGAATCTGCTG	TGCATCACCTCACATAGGTTA
DFNB2(MYO7A) 76839310..76926286) Hair bundle morphogenesis proteins: Motor proteins	D11S4179	76396260–76396495	200–256	GGATGTAAGAGTAACTGGCTCCG	GAAAATGTTCTGCCTGAGGG
	D11S4186	76968518–76968685	154–175	ATTCTCCCAATCTATCGCTC	GGGCAGTAATGATGATGTG
	D11S4079	77119447–77119701	217–265	CAGCAAGATCCTGTCTCAA	CTCCTTAAAGTGGGGGAGTT
	D11S911	77448583–77448769	159–203	CTTCTCATGCTTGACCATTT	CTTCTGAACAATTGCCACAT
DFNB3(MYO15A) 18012020..18083116) Hair bundle morphogenesis proteins: Motor proteins	D17S921	14260705–14260882	169–185	CTTGGACTCCTACAAATCCTGGCA	GGCCACCATAATCATGTCAGACAAT
	D17S953	16102497–16102619	119–131	ACTATCCGCCCAATACA	AAGGGCTTGCTTTGAC
	D17S2196	17264482–17264618	139–163	CCAACATCTAGAATTAATCAGAATC	ATATTTCAATATTGTAACCAGTCCC
DFNB4 (SLC26A4) 107301080..107358254) Ion homeostasis proteins: Ion channels	D7S2420	106889928–106890211	240–290	CCTGTATGGAGGGCAAACTA	AAATAATGACTGAGGCTCAAAACA
	D7S496	107154713–107154849	129–141	AACAACAGTCAACCCACAAT	GCTATAACCTCATAANAAACCAAAA
	D7S2459	107331501–107331642	140–152	AAGAAGTGCATTGAGACTCC	CCGCCTTAGTAAAACCC
	D7S2456	107683218–107683460	238–252	CTGGAAATTGACCTGAAACCTT	ACAGGGGTCTCTCACACATATTA
	D7S2425	108347079–108347322	234–246	CTAGTCCTGAGAAGACATTACCC	CCTGTTTCAGATGTTTTATCCA
DFNB6 (TMIE) 46742823..46752413) Poorly understood function: integral membrane protein	D3S3658	40903258–40903371	104–126	AAAAGTTAGCAAACACAATCCTATC	CTGGACTAAATCTAAGTTGGTTATG
	D3S2420	48067370–48067462	93–108	ACAAGTGCGAAACTCTGCCT	CAGGAGCCTCTAAGTCAGCA
DFNB7/11(TMC1 75136717..75451267) poorly understood function:integral membrane protein	D9S1837	75185129–75185367	205–251	CATGATGGTGGTCTCTGG	GGTGGGGCTCAAAGAGTAG
	D9S1806	74201357–74201620	216–266	TTTTAGGTGTTCTCAGTACATGC	GGGAGCAACATTTTGACATT
	D9S1124	75224065–75224327	252–276	GGTGCCCACCATACACTACT	TCTAATCCTTCCTTCCCTCG
	D9S1876	75232791–75232938	132–152	GATGTACCCAGAGAAGTCTCG	AGTGGTTACCATTTACCCAAG
	D9S301	73802720–73802954	209–237	AGTTTTCATAACACAAAAGAGAACA	ACCTAAATGTTCATCAAAAGAGG
	D9S1822	74930323–74930483	157–163	AAGTTTGGCTTCTGCTGTAAGGGTC	AATTCCCCCAGGCTGAGTG
	D9S1799	73366891–73367055	139–178	TTGCCAACTATTTTAGCCC	TGCAGTTTCAATCCACATC
DFNB8/10 (TMPRSS3) 43791999..43816200) Poorly understood function: Serine protease	D21S1890	44848178–44848330	143–173	GGTCTGACCACAGATTTCC	AAAAACACTCTGAACGATTAAGG
	D21S1260	42796042–42796251	200–214	TCCAAGGGGTTCATCC	CCCAAGGCACTGTTCC
DFNB9 (OTOF) 26680071..26781566) Poorly understood function: Exocytosis at auditory ribon synapse	D2S365	28606342–28606533	164–204	ATGATTTGTGTACCTTATGTATGTT	TCAATGGAGGAATCCTACTT
	D2S2247	27303911–27304064	130–160	TCCATCTTTTGCGTGC	CCGTGCTCTATGCCAG
	D2S174	26839873–26840075	203–221	AGGCTGAATCCCACCTCC	TTAGAGCACACATGGTCACTCC
	D2S2223	26559144–26559325	182–200	CACTGCGCCTAGCCTC	GGCGATTTATGAATAATCCTGC
DFNB12(CDH23) 73156704..73575704) Hair bundle morphogenesis proteins: Adhesion proteins	D10S1432	74659396–74659569	157–185	CAGTGGACACTAAACACAATCC	TAGATTATCTAAATGGTGGATTTCC
	D10S1146	74659314–74659555	164–246	ATTGCACTCCAGCCTGGGT	CACAATCCAATCACATGGATG
DFNB21(TECTA) 120973375..121061515) Extracellular matrix proteins:Structural component tectorial membrane	D11S4107	121049124–121049321	172–212	TCATTCTACAAGACTAGCATTACC	GCTTGATCATGGTGTATTATCTT
	D11S925	120828264–120828438	172–199	AGAACCAAGGTCGTAAGTCCTG	TTAGACCATTATGGGGGCAA
	D11S912	128624097–128624205	101–123	TCGTGAGANTACTGCTTTGG	TTTTGTCTAGCCATGATTGC
	D11S4151	126292160–126292309	145–155	GTCTTCCCACCTTGGATATGGGTA	AATGGGCACCTCCACCCTATTAGT
	D11S4089	120989673–120989875	199–213	ATTCCTAGTTCCCTCATAAACACTG	TAATCAAAGGCTGTAGTGAATTGG
	D11S4110	126971672–126971780	93–107	TGAGCCTCCCAGTACCTACC	GTTTGTGGCAGAGCCCTAAG
DFNB22(OTOA) 21689835..21772050) Extracellular matrix proteins: Anchoring protein between cellular gels and non-sensory cells	D16S3046	20886507–20886610	84–108	CCCAGAATAAACTGCGTG	TTCATGGACCCCCTATTG
	D16S403	23037651–23037790	134–152	GTTTTCTCCCTGGGACATTT	TATTCATTTGTGTGGGCATG
DFNB28 (TRIOBP) 38092995..38172563) Hair bundle morphogenesis proteins: Proteins of the cytoskeleton	D22S1156	38381771–38381926	130–162	TGAGGTAGTCACACGAGGCA	AATTCACTGGGCTCCGAGG
	D22S1045	37536298–37536453	140–158	GCTAGATTTTCCCCGATGAT	ATGTAAAGTGCTCTCAAGAGTGC
DFNB35(ESRRB) 76837726..76967208) Transcription factors	D14S1015	92736133–92736396	224–264	GAATGCCATTATTTTGTCCT	TTAGAAAACACCGAGCAGA
	D14S77	73570540–73570772	203–251	GCGTGAGTCACTGTGCC	CAGACAGAAATTAACCAGAGTTGAA
	D14S1045	0	240–246	AGGGCTGGTGACAATG	GTAAGGNCTTGGGTGG
	D14S48	88428727–88429001	260–265	CATAAAAGGCTTATTGGTTTG	CAAAACAGAGAACAGAGTAG
DFNB59 (PJVK) 179316163..179326113) Poorly understood function: Signaling of hair cells and neurons	D2S2981	176173334–176173578	234–262	AAAATATGCAGGTAATGACTTGG	CAAGCAAAACTGACAGGTAGG
	D2S301	217887163–217887396	224–240	CATAGGACTGAAGGGGTGTA	GGAAAATCTCGAATGTACCAAT
	D2S2173	178445536–178445768	201–243	GGAGACAGAGAGTTTACATTTGAG	GCCACACTTTCCTGAATC
	D2S324	179656244–179656508	264–275	TTACCCACCGGGACAGT	CAGCAAATGCTTCTAGGTCA
	D2S307	204654566–204654784	205–221	CATGACCTGAAATAAACATAGACA	AGCTTTTCCTGTAGGCTGTC
	D2S2314	176862406–176862505	96–118	GGTGTCAGTGAGACCCTGT	ATTTCTAGCGGCCCTAAAAC
DFNB63 (LRTOMT) 71791382..71821828) leucine-rich methyltransferase	D11S1314	72323192–72323414	209–227	TTGCTACGCACTCCTCTACT	GTGAAGGCAGGAAATGTGAC
	D11S4132	119948397–119948596	176–214	GTGCAAGTTTTGGCTTCGTC	ACTCCAGCCTGGGTGAAA
	D11S4162	70975752–70976016	263–269	GTTCTCCAGAGAGACAGCAC	GAGAGCAACACTATTGCCC
	D11S4140	71945684–71945874	189–199	TGCAACAAGGTTCCACACT	CTTATGGGTGAGGGCACAG
	D11S4196	0	200–240	GAACGTTNTTCATGTAGGCGT	TAATGGTCGCTGTCCC
	D11S4184	72670843–72671103	263–277	CCCAGCCTTACATATTCC	GCTGATGAGCAGAGGTAG

Physical positions were determined from the National Center for Biotechnology Information (NCBI) Build 37.1 sequence-based physical map (International Human Genome Sequence Consortium 2009).