Skip to main content
. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Apr 12;32(6):1427–1435. doi: 10.1161/ATVBAHA.111.241844

Figure 4. Dual-ligand MPIO binding quantitatively reflects lesion macrophage content.

Figure 4

(A) Representative images of aortic roots with lesion macrophage immunostained using Gal-3 antibody. Scale bar = 0.5 mm. Quantification of total lesion macrophage area (mm2) showed significant increases in lesion macrophage content from 8 to 20 wks and a significant reduction between 20 and 30 weeks. Magnification x 5. (B) Bound dual-ligand MPIO (yellow spheres) localized to endothelium overlying atherosclerotic lesions, with no binding observed to macrophages within the lesion. Magnification x 100. (C) Lesion size also increased from 8 to 20 weeks, but there was no change between 20 to 30 weeks. (D) Lesion macrophage content correlated significantly with dual-ligand MPIO MRI signal area.