Table 1.

Primary antibodies used in this study

Antibody	Species	Dilution	Antigen	Source, catalog no.	Specificity
CAVIII	Rabbit	1: 50	Epitope corresponding to amino acids 1–100 mapping at the N-terminus of CAVIII of human origin	Santa-Cruz Biotechnology, Santa Cruz, CA #67330, USA	See Fig. 1
PKC	Mouse	1: 400	Amino acids 296–317 of PKC	Sigma, St Louis, MO #P5704, USA	Recognizes an 80-kDa polypeptide of PKC in bovine brain lysates
RIBEYE (Ctbp2)	Mouse	1: 2000	Amino acids 361–445 (C-terminus) of mouse CtBP2	BD Biosciences Pharmingen, San Diego, CA #612044, USA	Recognizes a 48-kDa band on Western blots of mouse brain smooth muscle-like cells (manufacturer’s data sheet)
mGluR6	Guinea pig	1: 1000	Synthetic peptide corresponding to amino acids 853–871 of mouse mGluR6	Gift from Prof. Furukawa Takahisa, Osaka Bioscience Institute, Japan	Staining absent in mGluR6 knockout mouse (Koike et al., 2010)
SCGN	Sheep	1: 2000–1: 5000	276 amino acids (full length) of recombinant human SCGN and 10 extra AA, N-terminal His-tag	Biovendor R&D (#RD184120100)	Pattern obtained with this antibody is identical to a non-commercial antibody that recognizes a 32-kDa band on Western blot of mouse retina and brain lysates (Puthussery et al., 2010)
vGluT1	Guinea pig	1: 5000	Synthetic peptide (amino acids 542–560 of rat vGlut1)	Millipore #AB5905	Recognizes a 62-kDa band on Western blots of brain lysates (manufacturer’s data sheet)
Calretinin	Goat	1: 1000	Rat calretinin	Millipore #AB1550	Detects a ~30-kDa band on Western blots of rat brain extracts (Winsky et al., 1996)
GABA	Guinea pig	1: 5000	GABA coupled to KLH via glutaraldehyde	Millipore #AB175	Staining blocked by preadsorbing antibody with 100 μm GABA couples to glutaraldehyde (manufacturer’s data sheet)

CAVIII, carbonic anhydrase-related protein VIII; GABA, γ-aminobutyric acid; PKC, protein kinase C; SCGN, secretagogin; vGluT1, vesicular glutamate transporter 1.