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Northern analysis. Lane 1, Wild-type tobacco line; lanes 2 to 4, transgenic p35S-SMT2 plant lines A, D, and E, respectively. Ten micrograms of total leaf RNA from T3 plants was hybridized with a probe derived from the Arabidopsis SMT2. The arrow denotes the SMT2 1.4-kb transcript. The ethidium bromide-stained gel demonstrates equivalent RNA quantities loaded in each lane.
