Table I.
Total sterol content of tobacco plants transformed with an Arabidopsis SMT2 cDNA
| Sterola | Control
|
p35S-smt2
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Wild type | p35S | A | B | C | D | E | F | G | |
| % of total | |||||||||
| Cycloartenol | 2 | 2 | 2 | 8 | 3 | 2.5 | 2 | 3 | 2 |
| Cholesterol | 7 | 7 | 2 | 2 | 5 | 2 | 3 | 6 | 4 |
| 24-Methylene cholesterol | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
| 24-Methyl cholesterol | 22 | 22 | 1.5 | 0.5 | 10 | 1.5 | 1 | 5 | 5 |
| Isofucosterol | 9 | 9 | 6.5 | 5 | 11 | 7 | 6 | 6 | 9 |
| Sitosterol | 20 | 18 | 40 | 40.5 | 32 | 47 | 43 | 35 | 32 |
| Stigmasterol | 39 | 41 | 48 | 44 | 38 | 40 | 45 | 44 | 48 |
| mg g−1 dry wt | |||||||||
| Total amount | 2.2 | 2.1 | 2.1 | 1.9 | 1.8 | 2.3 | 2.8 | 1.9 | 2.1 |
| R | 1.10 | 1.22 | 0.04 | 0.01 | 0.31 | 0.03 | 0.02 | 0.14 | 0.15 |
Analysis was performed on leaf material from T1 plants grown for 6 weeks in the greenhouse.
a
β-Amyrin, cycloeucalenol, and Δ7-sitostenol were found in trace amounts in all samples.